adam10 (R&D Systems)
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adam10
Adam10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+adam10/Human+ADAM10+Ectodomain+Antibody/pm41926337-53-17-18
Average 91 stars, based on 12 article reviews
Adam10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+adam10/Human+ADAM10+Ectodomain+Antibody/pm41926337-53-17-18
Average 91 stars, based on 12 article reviews
adam10 - by Bioz Stars,
2026-09
91/100 stars
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Enzyme-linked Immunosorbent Assay:Article Title: Prostate cancer cells hyper-activate CXCR6 signaling by cleaving CXCL16 to overcome effect of docetaxel Article Snippet: Human CXCL16 was procured from Peprotech. .. PE-conjugated mouse anti-human CXCR6 antibody, APC-conjugated rat anti-human CXCL16, respective isotype controls (PE-conjugated IgG2b, APC-conjugated IgG2a), anti-phospho-RelA/NF-κB-p65 (Ser-529) , Blocking Assay:Article Title: Extracellular vesicles isolated from frozen and fresh human melanoma tissue are similar in purity and protein composition Article Snippet: The proteins were then transferred to PVDF membranes (Bio-Rad Laboratories) that were incubated in EveryBlot Blocking Buffer (Bio-Rad Laboratories) for 5 min at RT to block nonspecific binding. .. The following primary antibodies diluted in EveryBlot Blocking Buffer (Bio-Rad Laboratories) were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution, clone AB-2547893, Invitrogen), Membrane:Article Title: Extracellular vesicles isolated from frozen and fresh human melanoma tissue are similar in purity and protein composition Article Snippet: The proteins were then transferred to PVDF membranes (Bio-Rad Laboratories) that were incubated in EveryBlot Blocking Buffer (Bio-Rad Laboratories) for 5 min at RT to block nonspecific binding. .. The following primary antibodies diluted in EveryBlot Blocking Buffer (Bio-Rad Laboratories) were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution, clone AB-2547893, Invitrogen), Incubation:Article Title: Extracellular vesicles isolated from frozen and fresh human melanoma tissue are similar in purity and protein composition Article Snippet: The proteins were then transferred to PVDF membranes (Bio-Rad Laboratories) that were incubated in EveryBlot Blocking Buffer (Bio-Rad Laboratories) for 5 min at RT to block nonspecific binding. .. The following primary antibodies diluted in EveryBlot Blocking Buffer (Bio-Rad Laboratories) were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution, clone AB-2547893, Invitrogen), Article Title: Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples Article Snippet: .. After transferring to PVDF membranes (Bio‐Rad Laboratories, Hercules, CA, USA), the membranes were blocked with 5% Blotting Grade Blocker Non‐Fat Dry Milk in TBS containing 0.1% Tween‐20 (TBST) for 1 h at RT and then incubated with the following primary antibodies diluted in 2.5% Blotting Grade Blocker Non‐Fat Dry Milk in TBST at 4°C overnight: anti‐calnexin (1:1,000 dilution, clone C5C9, Cell Signalling Technology, Leiden, the Netherlands), anti‐flotillin‐1 (1:1,000 dilution, clone EPR6041, Abcam, Cambridge, UK), anti‐CD63 (1:1,000 dilution, clone H5C6, BD Biosciences), anti‐CD9 (1:1,000 dilution, clone MM2/57, Millipore, Darmstadt, Germany), anti‐CD81 (1:1,000 dilution, clone M38, Abcam), anti‐mitofilin (1:500 dilution, polyclonal, Invitrogen, Carlsbad, CA, USA), Article Title: EphA8 acts as an oncogene and contributes to poor prognosis in gastric cancer via regulation of ADAM10. Article Snippet: Department of Clinical Biobank, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China Department of Pathology, Medical School of Nantong University, Nantong, Jiangsu, China Department of Oncology, Medical School of Nantong University, Nantong, Jiangsu, China Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China Department of General Surgery, Medical School of Nantong University, Nantong, Jiangsu, China Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China Recombinant:Article Title: ADAM Sheddase Activity Promotes the Detachment of Small Extracellular Vesicles From the Plasma Membrane Article Snippet: The ERK1/2 inhibitor PD98059 (Calbiochem, 513001) was purchased as a ready‐to‐use 5 mg/mL solution in DMSO. .. The following primary antibodies were used: rabbit monoclonal recombinant anti‐CD63 [clone EPR21151 ] (Abcam, ab217345); mouse monoclonal anti‐CD63 [clone H5C6] (BD Bioscience, 556019); rabbit monoclonal anti‐CD81 (Invitrogen, MA5‐32333); rabbit polyclonal anti‐ADAM17 (Immunofluorescence, Biovision, 3222); rabbit polyclonal anti‐ADAM17 (western blot, QED Bioscience, 1131); rat monoclonal Immunofluorescence:Article Title: ADAM Sheddase Activity Promotes the Detachment of Small Extracellular Vesicles From the Plasma Membrane Article Snippet: The ERK1/2 inhibitor PD98059 (Calbiochem, 513001) was purchased as a ready‐to‐use 5 mg/mL solution in DMSO. .. The following primary antibodies were used: rabbit monoclonal recombinant anti‐CD63 [clone EPR21151 ] (Abcam, ab217345); mouse monoclonal anti‐CD63 [clone H5C6] (BD Bioscience, 556019); rabbit monoclonal anti‐CD81 (Invitrogen, MA5‐32333); rabbit polyclonal anti‐ADAM17 (Immunofluorescence, Biovision, 3222); rabbit polyclonal anti‐ADAM17 (western blot, QED Bioscience, 1131); rat monoclonal Western Blot:Article Title: ADAM Sheddase Activity Promotes the Detachment of Small Extracellular Vesicles From the Plasma Membrane Article Snippet: The ERK1/2 inhibitor PD98059 (Calbiochem, 513001) was purchased as a ready‐to‐use 5 mg/mL solution in DMSO. .. The following primary antibodies were used: rabbit monoclonal recombinant anti‐CD63 [clone EPR21151 ] (Abcam, ab217345); mouse monoclonal anti‐CD63 [clone H5C6] (BD Bioscience, 556019); rabbit monoclonal anti‐CD81 (Invitrogen, MA5‐32333); rabbit polyclonal anti‐ADAM17 (Immunofluorescence, Biovision, 3222); rabbit polyclonal anti‐ADAM17 (western blot, QED Bioscience, 1131); rat monoclonal Article Title: Polysaccharide enhanced NK cell cytotoxicity against pancreatic cancer via TLR4/MAPKs/NF-κB pathway in vitro/vivo. Article Snippet: A polysaccharide isolated from Strongylocentrotus nudus eggs (SEP) reportedly displays immune activity in vivo.. Here, its effect and underlying mechanism in the treatment of pancreatic cancer were investigated.. SEP obviously inhibited pancreatic cancer growth by activating NK cells in vitro/vivo via TLR4/MAPKs/NF-κB signaling pathway, The tumor inhibitory rate achieved to 44.5% and 50.8% at a dose of 40 mg/kg in Bxpc-3 and SW1990 nude mice, respectively. Transferring:Article Title: Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples Article Snippet: .. After transferring to PVDF membranes (Bio‐Rad Laboratories, Hercules, CA, USA), the membranes were blocked with 5% Blotting Grade Blocker Non‐Fat Dry Milk in TBS containing 0.1% Tween‐20 (TBST) for 1 h at RT and then incubated with the following primary antibodies diluted in 2.5% Blotting Grade Blocker Non‐Fat Dry Milk in TBST at 4°C overnight: anti‐calnexin (1:1,000 dilution, clone C5C9, Cell Signalling Technology, Leiden, the Netherlands), anti‐flotillin‐1 (1:1,000 dilution, clone EPR6041, Abcam, Cambridge, UK), anti‐CD63 (1:1,000 dilution, clone H5C6, BD Biosciences), anti‐CD9 (1:1,000 dilution, clone MM2/57, Millipore, Darmstadt, Germany), anti‐CD81 (1:1,000 dilution, clone M38, Abcam), anti‐mitofilin (1:500 dilution, polyclonal, Invitrogen, Carlsbad, CA, USA), Milk:Article Title: Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples Article Snippet: .. After transferring to PVDF membranes (Bio‐Rad Laboratories, Hercules, CA, USA), the membranes were blocked with 5% Blotting Grade Blocker Non‐Fat Dry Milk in TBS containing 0.1% Tween‐20 (TBST) for 1 h at RT and then incubated with the following primary antibodies diluted in 2.5% Blotting Grade Blocker Non‐Fat Dry Milk in TBST at 4°C overnight: anti‐calnexin (1:1,000 dilution, clone C5C9, Cell Signalling Technology, Leiden, the Netherlands), anti‐flotillin‐1 (1:1,000 dilution, clone EPR6041, Abcam, Cambridge, UK), anti‐CD63 (1:1,000 dilution, clone H5C6, BD Biosciences), anti‐CD9 (1:1,000 dilution, clone MM2/57, Millipore, Darmstadt, Germany), anti‐CD81 (1:1,000 dilution, clone M38, Abcam), anti‐mitofilin (1:500 dilution, polyclonal, Invitrogen, Carlsbad, CA, USA), |
